Hotspot

psbA-trnH-GUG

LSC · 1290-1553 · 264 bp

Back to Passiflora

Pi 0.0524
MarkerSeek score 85.80
Primer available yes
Label psbA-trnH-GUG

Feature Metadata

label_name
psbA-trnH-GUG
feature_type
igs
region
LSC
length_bp
264
variable_sites
71
pi
0.0524
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnH-GUG_p1 CCTCTAGACCTAGCTGCTGT CAATCCACCGCCTTTATCCA 55.0 50.0 57.9 57.9 296-354 bp 1.0 91.1
psbA-trnH-GUG_p2 CCTCTAGACCTAGCTGCTGT AATCCACCGCCTTTATCCAC 55.0 50.0 57.9 57.9 295-353 bp 1.0 91.1
psbA-trnH-GUG_p3 CCTCTAGACCTAGCTGCTGT ACAATCCACCGCCTTTATCC 55.0 50.0 57.9 57.9 297-355 bp 1.0 91.1
psbA-trnH-GUG_p4 TTCCCTCTAGACCTAGCTGC CAATCCACCGCCTTTATCCA 55.0 50.0 57.9 57.9 299-357 bp 1.0 91.0
psbA-trnH-GUG_p5 TTCCCTCTAGACCTAGCTGC AATCCACCGCCTTTATCCAC 55.0 50.0 57.9 57.9 298-356 bp 1.0 91.0