Hotspot

trnL-CAA-trnI-CAU

IRa · 134299-139482 · 5184 bp

Back to Paspalum

Pi 0.126

MarkerSeek score 79.30

Primer available yes

Label trnL-CAA-trnI-CAU

Feature Metadata

label_name: trnL-CAA-trnI-CAU
feature_type: igs
region: IRa
length_bp: 5184
variable_sites: 1495
pi: 0.126
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-trnI-CAU_p1	TGAAACGTGGCGATGATGAT	AGTCTCCCCTCTCTCCCTTA	45.0	55.0	58.0	58.0	428 bp	0.173913	56.6
trnL-CAA-trnI-CAU_p2	CTGAGACGAAGAGAAAGGGC	AGTCTCCCCTCTCTCCCTTA	55.0	55.0	58.0	58.0	763 bp	0.130435	45.2
trnL-CAA-trnI-CAU_p3	CTGAGACGAAGAGAAAGGGC	CGCTACGGATATCCTTCAGC	55.0	55.0	58.0	58.0	918 bp	0.130435	45.1
trnL-CAA-trnI-CAU_p4	CTGAGACGAAGAGAAAGGGC	TAGAGATGGACTACGGGCAG	55.0	55.0	58.0	58.0	661 bp	0.130435	45.1
trnL-CAA-trnI-CAU_p5	CTGAGACGAAGAGAAAGGGC	TTTCGCTAGCTCACATGCAT	55.0	45.0	58.0	58.0	953 bp	0.130435	45.0