Hotspot

trnI-CAU-trnL-CAA

IRb · 83778-88961 · 5184 bp

Back to Paspalum

Pi 0.135

MarkerSeek score 79.10

Primer available yes

Label trnI-CAU-trnL-CAA

Feature Metadata

label_name: trnI-CAU-trnL-CAA
feature_type: igs
region: IRb
length_bp: 5184
variable_sites: 1558
pi: 0.135
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-trnL-CAA_p1	TCTCAGACAGTAGTGCCACA	TGAAACGTGGCGATGATGAT	50.0	45.0	58.0	58.0	1129 bp	0.173913	57.9
trnI-CAU-trnL-CAA_p2	AGTCTCCCCTCTCTCCCTTA	TGAAACGTGGCGATGATGAT	55.0	45.0	58.0	58.0	428 bp	0.173913	57.4
trnI-CAU-trnL-CAA_p3	AGTCTCCCCTCTCTCCCTTA	CTGAGACGAAGAGAAAGGGC	55.0	55.0	58.0	58.0	763 bp	0.130435	45.2
trnI-CAU-trnL-CAA_p4	TCTCAGACAGTAGTGCCACA	CTGAGACGAAGAGAAAGGGC	50.0	55.0	58.0	58.0	1464 bp	0.130435	45.2
trnI-CAU-trnL-CAA_p5	CGCTACGGATATCCTTCAGC	CTGAGACGAAGAGAAAGGGC	55.0	55.0	58.0	58.0	918 bp	0.130435	45.1