Hotspot

ycf3-trnS-GGA

LSC · 45729-46332 · 604 bp

Back to Paspalum

Pi 0.00809

MarkerSeek score 66.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 604
variable_sites: 35
pi: 0.00809
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTGGCTACAATCGAGGAGGT	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.4	57.8	681-728 bp	1.0	82.1
ycf3-trnS-GGA_p2	TTGGCTACAATCGAGGAGGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.4	58.2	731-778 bp	1.0	81.9
ycf3-trnS-GGA_p3	GGCTACAATCGAGGAGGTCT	TTCCAATGCTACGCCTTGAA	55.0	45.0	58.6	57.8	679-726 bp	1.0	80.8
ycf3-trnS-GGA_p4	GGCTACAATCGAGGAGGTCT	ACGGAAAGAGAGGGATTCGA	55.0	50.0	58.6	58.2	729-776 bp	1.0	80.6
ycf3-trnS-GGA_p5	TTGGCTACAATCGAGGAGGT	AGTTCCAATGCTACGCCTTG	50.0	50.0	58.4	58.5	683-730 bp	1.0	79.2