Hotspot

trnD-GUC-psbM

LSC · 17030-18107 · 1078 bp

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Pi 0.0122
MarkerSeek score 67.70
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1078
variable_sites
84
pi
0.0122
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG TCATTTTGGCTGGCTGTTTT 50.0 40.0 58.5 56.7 773-1249 bp 1.0 75.3
trnD-GUC-psbM_p2 TTCAATTGGTCAGAGCACCG AATCATTTTGGCTGGCTGTT 50.0 40.0 58.5 56.5 775-1251 bp 1.0 73.5
trnD-GUC-psbM_p3 TTCAATTGGTCAGAGCACCG ATCATTTTGGCTGGCTGTTT 50.0 40.0 58.5 56.5 774-1250 bp 1.0 73.5
trnD-GUC-psbM_p4 TTCAATTGGTCAGAGCACCG TCATTTTGGCTGGCTGTTTTT 50.0 38.1 58.5 57.4 773-1249 bp 1.0 73.3
trnD-GUC-psbM_p5 TTCAATTGGTCAGAGCACCG TTTGGCTGGCTGTTTTTACA 50.0 40.0 58.5 56.3 769-1245 bp 1.0 72.5