Hotspot

trnK-UUU-rps16

LSC · 3916-4465 · 550 bp

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Pi 0.0115

MarkerSeek score 65.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 550
variable_sites: 42
pi: 0.0115
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CATTGAGTTAGCAACCCGGA	TCTTGAAAAGGGGGCTCAAC	50.0	50.0	57.9	58.0	663-670 bp	1.0	84.7
trnK-UUU-rps16_p2	ACCATTGAGTTAGCAACCCG	TCTTGAAAAGGGGGCTCAAC	50.0	50.0	58.2	58.0	665-672 bp	1.0	84.2
trnK-UUU-rps16_p3	CATTGAGTTAGCAACCCGGA	GAAAAGGGGGCTCAACCTAC	50.0	55.0	57.9	58.2	659-666 bp	1.0	83.5
trnK-UUU-rps16_p4	CATTGAGTTAGCAACCCGGA	GGGGCTCAACCTACAAGAAC	50.0	55.0	57.9	58.2	653-660 bp	1.0	83.5
trnK-UUU-rps16_p5	ACCATTGAGTTAGCAACCCG	GAAAAGGGGGCTCAACCTAC	50.0	55.0	58.2	58.2	661-668 bp	1.0	82.9