Hotspot

rpl2

IRa · 159855-161339 · 1485 bp

Back to Parthenocissus

Pi 0.000898
MarkerSeek score 59.30
Primer available yes
Label rpl2

Feature Metadata

label_name
rpl2
feature_type
gene
region
IRa
length_bp
1485
variable_sites
2
pi
0.000898
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2_p1 ACGTGTCACAGTTAATTACTCCT CGCTTCAACCGGGTTATTCT 39.1 50.0 57.5 58.3 1631-1634 bp 1.0 50.4
rpl2_p2 ACGTGTCACAGTTAATTACTCCT ATCATTACGCTTCAACCGGG 39.1 50.0 57.5 58.3 1638-1641 bp 1.0 49.8
rpl2_p3 ACGTGTCACAGTTAATTACTCCT ACGCTTCAACCGGGTTATTC 39.1 50.0 57.5 58.6 1632-1635 bp 1.0 48.1
rpl2_p4 ACGTGTCACAGTTAATTACTCC CGCTTCAACCGGGTTATTCT 40.9 50.0 56.1 58.3 1631-1634 bp 1.0 47.3
rpl2_p5 ACGTGTCACAGTTAATTACTCC ATCATTACGCTTCAACCGGG 40.9 50.0 56.1 58.3 1638-1641 bp 1.0 46.7
rpl2_p1 CGCTTCAACCGGGTTATTCT ACGTGTCACAGTTAATTACTCCT 50.0 39.1 58.3 57.5 1631-1634 bp 1.0 50.4
rpl2_p2 ATCATTACGCTTCAACCGGG ACGTGTCACAGTTAATTACTCCT 50.0 39.1 58.3 57.5 1638-1641 bp 1.0 49.8
rpl2_p3 ACGCTTCAACCGGGTTATTC ACGTGTCACAGTTAATTACTCCT 50.0 39.1 58.6 57.5 1632-1635 bp 1.0 48.1
rpl2_p4 CGCTTCAACCGGGTTATTCT ACGTGTCACAGTTAATTACTCC 50.0 40.9 58.3 56.1 1631-1634 bp 1.0 47.3
rpl2_p5 ATCATTACGCTTCAACCGGG ACGTGTCACAGTTAATTACTCC 50.0 40.9 58.3 56.1 1638-1641 bp 1.0 46.7