Hotspot

rbcL

LSC · 59685-61112 · 1428 bp

Back to Parthenocissus

Pi 0.007

MarkerSeek score 44.40

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 15
pi: 0.007
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAGTTGTAGGGAGGGACTT	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.3	58.4	1500 bp	1.0	72.0
rbcL_p2	TGAGTTGTAGGGAGGGACTT	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.3	58.4	1501 bp	1.0	72.0
rbcL_p3	TTCGTGAGTTGTAGGGAGGG	TGGGCCGAGTTTAATTGCAA	55.0	45.0	58.7	58.4	1504 bp	1.0	71.9
rbcL_p4	TTCGTGAGTTGTAGGGAGGG	TTGGGCCGAGTTTAATTGCA	55.0	45.0	58.7	58.4	1505 bp	1.0	71.9
rbcL_p5	CGTGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	57.9	45.0	58.0	58.4	1502 bp	1.0	69.5