Hotspot

psaB

LSC · 40978-43182 · 2205 bp

Back to Parthenocissus

Pi 0.00151

MarkerSeek score 58.20

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 5
pi: 0.00151
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	TCGCGGATACAGCACATTAA	ACAACATGGGCGTTCTTCTT	45.0	45.0	57.4	58.0	2308 bp	1.0	75.3
psaB_p2	TCGCGGATACAGCACATTAA	TACAAGGACGTGCTGTAGGA	45.0	50.0	57.4	58.1	2358 bp	1.0	74.8
psaB_p3	TCGCGGATACAGCACATTAA	GGGCGTTCTTCTTAGCAAGA	45.0	50.0	57.4	57.9	2301 bp	1.0	74.7
psaB_p4	TCGCGGATACAGCACATTAA	TGGGCGTTCTTCTTAGCAAG	45.0	50.0	57.4	58.2	2302 bp	1.0	73.9
psaB_p5	TCGCGGATACAGCACATTAA	AGGACGTGCTGTAGGAGTAA	45.0	50.0	57.4	57.8	2354 bp	1.0	73.8