Hotspot

clpP

LSC · 75007-77049 · 2043 bp

Back to Parthenocissus

Pi 0.00718

MarkerSeek score 52.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2043
variable_sites: 22
pi: 0.00718
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCACGGATTTGCACGAAATC	ACGTTTCCATACCAAAGTGA	45.0	40.0	57.1	54.9	2115-2126 bp	1.0	49.8
clpP_p2	TCACGGATTTGCACGAAATC	ACGTTTCCATACCAAAGTGAA	45.0	38.1	57.1	55.7	2115-2126 bp	1.0	48.4
clpP_p3	TCACGGATTTGCACGAAATC	ACGTTTCCATACCAAAGTGAAA	45.0	36.4	57.1	56.5	2115-2126 bp	1.0	46.4
clpP_p4	AAATCACGGATTTGCACGAA	ACGTTTCCATACCAAAGTGA	40.0	40.0	56.6	54.9	2118-2129 bp	1.0	46.0
clpP_p5	AATCACGGATTTGCACGAAA	ACGTTTCCATACCAAAGTGA	40.0	40.0	56.6	54.9	2117-2128 bp	1.0	46.0