Hotspot

ndhF-rpl32

SSC · 118094-119303 · 1210 bp

Back to Parthenocissus

Pi 0.0116

MarkerSeek score 51.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1210
variable_sites: 21
pi: 0.0116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGGAAGTGGAAGGAAAGGT	ATTTCGCTAACGCAAAAGCC	45.0	45.0	56.6	58.0	1371 bp	1.0	69.6
ndhF-rpl32_p2	CCACATACGCCGAAGATTTT	ATTTCGCTAACGCAAAAGCC	45.0	45.0	56.5	58.0	1439 bp	1.0	69.1
ndhF-rpl32_p3	TTGGAAGTGGAAGGAAAGGT	CCACCATCCCTTCCTTTTCC	45.0	55.0	56.6	58.1	1340 bp	1.0	68.6
ndhF-rpl32_p4	TTGGAAGTGGAAGGAAAGGT	CCCACCATCCCTTCCTTTTC	45.0	55.0	56.6	58.1	1341 bp	1.0	68.6
ndhF-rpl32_p5	CCACATACGCCGAAGATTTT	CCACCATCCCTTCCTTTTCC	45.0	55.0	56.5	58.1	1408 bp	1.0	68.2