Hotspot

rps19-rpl2

IRb · 89362-89433 · 72 bp

Back to Parthenocissus

Pi 0
MarkerSeek score 57.00
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
IRb
length_bp
72
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 GATGTCCGGGACCAAGTTAC CACAACCCCTTGGGGTTATC 55.0 55.0 58.0 58.2 276-279 bp 1.0 79.5
rps19-rpl2_p2 AGATGTCCGGGACCAAGTTA CACAACCCCTTGGGGTTATC 50.0 55.0 58.1 58.2 277-280 bp 1.0 79.2
rps19-rpl2_p3 TAGATGTCCGGGACCAAGTT CACAACCCCTTGGGGTTATC 50.0 55.0 58.1 58.2 278-281 bp 1.0 79.2
rps19-rpl2_p4 GATGTCCGGGACCAAGTTAC TTGGGGTTATCCTGCACTTG 55.0 50.0 58.0 57.8 267-270 bp 1.0 79.0
rps19-rpl2_p5 AGATGTCCGGGACCAAGTTA TTGGGGTTATCCTGCACTTG 50.0 50.0 58.1 57.8 268-271 bp 1.0 78.8