Hotspot

psbH-petD

LSC · 79831-82167 · 2337 bp

Back to Parthenocissus

Pi 0.00542

MarkerSeek score 59.20

Primer available yes

Label psbH-petD

Feature Metadata

label_name: psbH-petD
feature_type: igs
region: LSC
length_bp: 2337
variable_sites: 19
pi: 0.00542
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petD_p1	CTCCTTTAATGGGTGTCGCA	ACAGGATCGTTCAAGTCAGG	50.0	50.0	57.9	57.2	2521-2522 bp	1.0	73.7
psbH-petD_p2	CTCCTTTAATGGGTGTCGCA	CAGGATCGTTCAAGTCAGGT	50.0	50.0	57.9	57.2	2520-2521 bp	1.0	73.7
psbH-petD_p3	ACTCCTTTAATGGGTGTCGC	ACAGGATCGTTCAAGTCAGG	50.0	50.0	57.9	57.2	2522-2523 bp	1.0	73.7
psbH-petD_p4	ACTCCTTTAATGGGTGTCGC	CAGGATCGTTCAAGTCAGGT	50.0	50.0	57.9	57.2	2521-2522 bp	1.0	73.7
psbH-petD_p5	GTCGCAATGGCTCTATTTGC	CAGGATCGTTCAAGTCAGGT	50.0	50.0	57.9	57.2	2506-2507 bp	1.0	73.7