Hotspot

petA-psbJ

LSC · 67800-68860 · 1061 bp

Back to Parthenocissus

Pi 0.00754

MarkerSeek score 37.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1061
variable_sites: 12
pi: 0.00754
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTGACGGGAGCAAGAGTAAC	ACACAATCAAACCCGAACGA	55.0	45.0	58.0	58.0	1824-1825 bp	1.0	80.4
petA-psbJ_p2	CTGACGGGAGCAAGAGTAAC	ACGACACAATCAAACCCGAA	55.0	45.0	58.0	58.0	1827-1828 bp	1.0	80.4
petA-psbJ_p3	CTGACGGGAGCAAGAGTAAC	GAGCCGACAAGGAATTCCAT	55.0	50.0	58.0	58.0	2057-2058 bp	1.0	80.3
petA-psbJ_p4	CTGACGGGAGCAAGAGTAAC	ACGGACTAGCTGTACCTACC	55.0	55.0	58.0	58.0	1917-1918 bp	1.0	80.3
petA-psbJ_p5	CTGACGGGAGCAAGAGTAAC	GGACTAGCTGTACCTACCGT	55.0	55.0	58.0	58.0	1915-1916 bp	1.0	80.3