Hotspot

trnF-GAA-ndhJ

LSC · 52419-53127 · 709 bp

Back to Parthenocissus

Pi 0.00658

MarkerSeek score 56.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 7
pi: 0.00658
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TAATCATCCACGCCTGAAGC	55.0	50.0	58.0	58.0	887-891 bp	1.0	80.3
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	850-854 bp	1.0	80.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TAATCATCCACGCCTGAAGC	50.0	50.0	58.1	58.0	851-855 bp	1.0	80.0
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	814-818 bp	1.0	79.7
trnF-GAA-ndhJ_p5	ATGATGCATCGGGAATAGCC	TAATCATCCACGCCTGAAGC	50.0	50.0	57.9	58.0	907-911 bp	1.0	79.4