Hotspot

psbZ-trnfM-CAU

LSC · 39713-40297 · 585 bp

Back to Parthenocissus

Pi 0.0182
MarkerSeek score 58.30
Primer available yes
Label psbZ-trnfM-CAU

Feature Metadata

label_name
psbZ-trnfM-CAU
feature_type
igs
region
LSC
length_bp
585
variable_sites
16
pi
0.0182
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnfM-CAU_p1 TTGCTTCTCCTGATGGTTGG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 729-1032 bp 1.0 80.0
psbZ-trnfM-CAU_p2 GCTTCTCCTGATGGTTGGTC CTTGAGGTCACGGGTTCAAA 55.0 50.0 58.3 58.0 727-1030 bp 1.0 79.6
psbZ-trnfM-CAU_p3 TTGCTTCTCCTGATGGTTGG GTCACGGGTTCAAATCCTGT 50.0 50.0 57.8 58.1 723-1026 bp 1.0 79.4
psbZ-trnfM-CAU_p4 GCTTCTCCTGATGGTTGGTC GTCACGGGTTCAAATCCTGT 55.0 50.0 58.3 58.1 721-1024 bp 1.0 79.0
psbZ-trnfM-CAU_p5 TTGCTTCTCCTGATGGTTGG TCACGGGTTCAAATCCTGTC 50.0 50.0 57.8 57.8 722-1025 bp 1.0 78.9