Hotspot

trnE-UUC-psbD

LSC · 34188-36391 · 2204 bp

Back to Parthenocissus

Pi 0.00817

MarkerSeek score 62.60

Primer available yes

Label trnE-UUC-psbD

Feature Metadata

label_name: trnE-UUC-psbD
feature_type: igs
region: LSC
length_bp: 2204
variable_sites: 27
pi: 0.00817
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-psbD_p1	TCCTTTCTTCCTACCCCCAG	TCGCTTTCGTCTTTGGTGAA	55.0	45.0	58.0	58.1	2328-2365 bp	1.0	80.3
trnE-UUC-psbD_p2	TCCTTTCTTCCTACCCCCAG	ATCGCTTTCGTCTTTGGTGA	55.0	45.0	58.0	57.8	2329-2366 bp	1.0	79.4
trnE-UUC-psbD_p3	TCCTTTCTTCCTACCCCCAG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.0	58.2	2379-2416 bp	1.0	78.7
trnE-UUC-psbD_p4	TCCTTTCTTCCTACCCCCAG	GACCGGACCAACCTACAAAA	55.0	50.0	58.0	57.7	2393-2430 bp	1.0	78.6
trnE-UUC-psbD_p5	GCTGCCTCCTTGAAAGAGAG	TCGCTTTCGTCTTTGGTGAA	55.0	45.0	58.3	58.1	2292-2329 bp	1.0	78.4