Hotspot

psbM-trnD-GUC

LSC · 32290-33470 · 1181 bp

Back to Parthenocissus

Pi 0.013

MarkerSeek score 64.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1181
variable_sites: 23
pi: 0.013
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TGGGCTATGGCTATACACCT	45.0	50.0	55.8	57.6	1313-1332 bp	1.0	62.2
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1274-1293 bp	1.0	61.8
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	GTGGGCTATGGCTATACACC	45.0	55.0	55.8	57.5	1314-1333 bp	1.0	61.7
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	TGGGCTATGGCTATACACCT	40.9	50.0	57.4	57.6	1320-1339 bp	1.0	59.0
psbM-trnD-GUC_p5	AGGAACTAGAATGAACAGTGCA	GTGGGCTATGGCTATACACC	40.9	55.0	57.4	57.5	1321-1340 bp	1.0	58.5