Hotspot

atpF-atpI

LSC · 14842-16436 · 1595 bp

Back to Parthenocissus

Pi 0.00794

MarkerSeek score 56.70

Primer available yes

Label atpF-atpI

Feature Metadata

label_name: atpF-atpI
feature_type: igs
region: LSC
length_bp: 1595
variable_sites: 19
pi: 0.00794
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpI_p1	CAGTGACCCAAGGAAACGAA	CGAATCCATGGAGGGTCATC	50.0	55.0	58.0	57.8	1661-1674 bp	1.0	79.8
atpF-atpI_p2	CAGTGACCCAAGGAAACGAA	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.0	58.5	1692-1705 bp	1.0	77.1
atpF-atpI_p3	TATTAAACCCGAAACCCCCG	CGAATCCATGGAGGGTCATC	50.0	55.0	57.6	57.8	1690-1703 bp	1.0	76.8
atpF-atpI_p4	AGTGACCCAAGGAAACGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	57.3	57.8	1660-1673 bp	1.0	74.4
atpF-atpI_p5	TATTAAACCCGAAACCCCCG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.6	58.5	1721-1734 bp	1.0	74.0