Hotspot

trnS-GCU-trnR-UCU

LSC · 9849-11707 · 1859 bp

Back to Parthenocissus

Pi 0.00538

MarkerSeek score 61.80

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1859
variable_sites: 15
pi: 0.00538
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ATTAGCAATCCGCCGCTTTA	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.0	56.9	1935-2005 bp	1.0	64.5
trnS-GCU-trnR-UCU_p2	ATTAGCAATCCGCCGCTTTA	AGGTTTAGAAGACCTCTGTCCT	45.0	45.5	58.0	57.9	1941-2011 bp	1.0	63.9
trnS-GCU-trnR-UCU_p3	ACGGAAAGAGAGGGATTCGA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.2	56.9	1984-2054 bp	1.0	63.6
trnS-GCU-trnR-UCU_p4	ACGGAAAGAGAGGGATTCGA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.2	57.9	1990-2060 bp	1.0	62.9
trnS-GCU-trnR-UCU_p5	TGGAAACGGAAAGAGAGGGA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.3	56.9	1989-2059 bp	1.0	62.6