Hotspot

trnK-UUU-rps16

LSC · 4316-5264 · 949 bp

Back to Parthenocissus

Pi 0.0119

MarkerSeek score 71.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 949
variable_sites: 17
pi: 0.0119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAGGAGCTCAACCTACAGGA	50.0	50.0	58.2	57.7	1076-1084 bp	1.0	80.3
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	AGGAGCTCAACCTACAGGAA	50.0	50.0	58.2	57.7	1075-1083 bp	1.0	80.3
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	AAGGAGCTCAACCTACAGGA	50.0	50.0	58.2	57.7	1075-1083 bp	1.0	80.3
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	AGGAGCTCAACCTACAGGAA	50.0	50.0	58.2	57.7	1074-1082 bp	1.0	80.3
trnK-UUU-rps16_p5	CAGTCGCGGTCTTACAAACT	AGGAGCTCAACCTACAGGAA	50.0	50.0	58.2	57.7	1159-1167 bp	1.0	79.6