Hotspot

rpl32-ndhF

SSC · 123774-124866 · 1093 bp

Back to Parthenium

Pi 0.0466

MarkerSeek score 80.40

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1093
variable_sites: 48
pi: 0.0466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TCCAAATATTTTTACGAATACGCT	GTTGCCGTCGGAAAAAGAAG	29.2	50.0	55.3	58.0	1217-1252 bp	1.0	47.1
rpl32-ndhF_p2	TCCAAATATTTTTACGAATACGCT	TGCCGTCGGAAAAAGAAGAA	29.2	45.0	55.3	58.0	1215-1250 bp	1.0	47.1
rpl32-ndhF_p3	TCCAAATATTTTTACGAATACGCT	TTGCCGTCGGAAAAAGAAGA	29.2	45.0	55.3	58.0	1216-1251 bp	1.0	47.1
rpl32-ndhF_p4	TCCAAATATTTTTACGAATACGCT	CTTTTTGTTGCCGTCGGAAA	29.2	45.0	55.3	57.8	1223-1258 bp	1.0	47.1
rpl32-ndhF_p5	TCCAAATATTTTTACGAATACGCT	TTTGTTGCCGTCGGAAAAAG	29.2	45.0	55.3	57.8	1220-1255 bp	1.0	47.1