Hotspot

ndhD-ccsA

SSC · 121670-121910 · 241 bp

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Pi 0.0407

MarkerSeek score 72.20

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 241
variable_sites: 9
pi: 0.0407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	AGGTATTGGTATGTACCCCGA	TGGCGACTCTAGGCTTTCTT	47.6	50.0	57.7	58.7	421-430 bp	1.0	69.2
ndhD-ccsA_p2	AGGTATTGGTATGTACCCCGA	CAATTGTGGCGACTCTAGGC	47.6	55.0	57.7	59.0	427-436 bp	1.0	67.1
ndhD-ccsA_p3	AGGTATTGGTATGTACCCCGA	GCAATTGTGGCGACTCTAGG	47.6	55.0	57.7	59.0	428-437 bp	1.0	67.1
ndhD-ccsA_p4	AGGTATTGGTATGTACCCCGA	TGGCGACTCTAGGCTTTCT	47.6	52.6	57.7	58.0	421-430 bp	1.0	66.8
ndhD-ccsA_p5	TCAGTTGACAAGGTTGAAGGT	TGGCGACTCTAGGCTTTCTT	42.9	50.0	57.4	58.7	384-393 bp	1.0	66.8