Hotspot

ndhI-ndhG

SSC · 118145-118514 · 370 bp

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Pi 0.0676

MarkerSeek score 70.20

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 370
variable_sites: 25
pi: 0.0676
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	TTGATCACATCCGAGCGTTT	AGACCACGGAAGTTACTCCA	45.0	50.0	57.9	58.0	1037-1069 bp	1.0	81.5
ndhI-ndhG_p2	TTGATCACATCCGAGCGTTT	AGACTTGTACAGACCACGGA	45.0	50.0	57.9	58.0	1047-1079 bp	1.0	81.4
ndhI-ndhG_p3	TGTGAAGTATGCGTTCGTGT	AGACCACGGAAGTTACTCCA	45.0	50.0	57.9	58.0	977-1009 bp	1.0	81.2
ndhI-ndhG_p4	GCTTGTGAAGTATGCGTTCG	AGACCACGGAAGTTACTCCA	50.0	50.0	57.9	58.0	980-1012 bp	1.0	81.2
ndhI-ndhG_p5	TGTGAAGTATGCGTTCGTGT	AGACTTGTACAGACCACGGA	45.0	50.0	57.9	58.0	987-1019 bp	1.0	81.2