Hotspot

psaJ-rpl33

LSC · 67505-67954 · 450 bp

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Pi 0.039

MarkerSeek score 69.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 17
pi: 0.039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCATTGACGTTCCCCTTT	AGAACCGGTATTCGCACATC	45.0	50.0	58.1	58.1	511-514 bp	1.0	83.7
psaJ-rpl33_p2	ATGCATTGACGTTCCCCTTT	GAACCGGTATTCGCACATCT	45.0	50.0	58.1	58.1	510-513 bp	1.0	83.7
psaJ-rpl33_p3	ACATATCTTTCCGTGGCACC	AGAACCGGTATTCGCACATC	50.0	50.0	58.0	58.1	599-602 bp	1.0	83.3
psaJ-rpl33_p4	ACATATCTTTCCGTGGCACC	GAACCGGTATTCGCACATCT	50.0	50.0	58.0	58.1	598-601 bp	1.0	83.3
psaJ-rpl33_p5	ACATATCTTTCCGTGGCACC	TCGCACATCTTTACCCTTGG	50.0	50.0	58.0	57.9	588-591 bp	1.0	83.0