Hotspot

trnS-GCU-psbM

LSC · 8395-10778 · 2384 bp

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Pi 0.164

MarkerSeek score 83.90

Primer available yes

Label trnS-GCU-psbM

Feature Metadata

label_name: trnS-GCU-psbM
feature_type: igs
region: LSC
length_bp: 2384
variable_sites: 344
pi: 0.164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbM_p1	CTTTCGTCCACTCAGCCATC	ACGTAAAAACAGCCAGTCAA	55.0	40.0	58.6	55.8	2287-2438 bp	1.0	69.5
trnS-GCU-psbM_p2	CTTTCGTCCACTCAGCCATC	ACGTAAAAACAGCCAGTCAAA	55.0	38.1	58.6	56.6	2287-2438 bp	1.0	67.6
trnS-GCU-psbM_p3	CTTTCGTCCACTCAGCCATC	TGCTACTGCACTGTTCATTT	55.0	40.0	58.6	55.6	2337-2488 bp	1.0	67.5
trnS-GCU-psbM_p4	TTTCGTCCACTCAGCCATCT	ACGTAAAAACAGCCAGTCAA	50.0	40.0	59.0	55.8	2286-2437 bp	1.0	66.4
trnS-GCU-psbM_p5	CTTTCGTCCACTCAGCCATC	TGCTACTGCACTGTTCATTTT	55.0	38.1	58.6	56.4	2337-2488 bp	1.0	65.8