Hotspot

trnH-psbA

LSC · 75-405 · 331 bp

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Pi 0.0547

MarkerSeek score 74.80

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 331
variable_sites: 18
pi: 0.0547
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	GATCCACTTGGCTACATCCG	TCCCTCTAGACTTAGCTGCT	55.0	50.0	58.1	56.9	400-508 bp	1.0	73.4
trnH-psbA_p2	CAATCCACTGCCTTGATCCA	TCCCTCTAGACTTAGCTGCT	50.0	50.0	57.9	56.9	414-522 bp	1.0	73.3
trnH-psbA_p3	AATCCACTGCCTTGATCCAC	TCCCTCTAGACTTAGCTGCT	50.0	50.0	57.9	56.9	413-521 bp	1.0	73.3
trnH-psbA_p4	ACAATCCACTGCCTTGATCC	TCCCTCTAGACTTAGCTGCT	50.0	50.0	57.9	56.9	415-523 bp	1.0	73.3
trnH-psbA_p5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACTTAGCTGCT	55.0	47.6	58.1	57.6	401-509 bp	1.0	71.4