Hotspot

ndhF

SSC · 126601-128841 · 2241 bp

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Pi 0.488
MarkerSeek score 78.80
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2241
variable_sites
908
pi
0.488
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACGTTTGAAATCCGTACGAGA ATTCAACAGGCTCTGGAAGG 42.9 50.0 57.7 57.5 2947 bp 0.5 45.7
ndhF_p2 ACGTTTGAAATCCGTACGAGA AACAGGCTCTGGAAGGACTT 42.9 50.0 57.7 58.6 2943 bp 0.5 45.2
ndhF_p3 ACGTTTGAAATCCGTACGAGA TTCAACAGGCTCTGGAAGGA 42.9 50.0 57.7 58.6 2946 bp 0.5 45.2
ndhF_p4 ACGTTTGAAATCCGTACGAG ATTCAACAGGCTCTGGAAGG 45.0 50.0 56.2 57.5 2947 bp 0.5 41.7
ndhF_p5 ACGTTTGAAATCCGTACGAGA ACAGGCTCTGGAAGGACTTC 42.9 55.0 57.7 59.0 2942 bp 0.5 41.5