Hotspot

ndhD

SSC · 122172-123614 · 1443 bp

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Pi 0.455
MarkerSeek score 80.30
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1443
variable_sites
547
pi
0.455
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 GCGTCGGTTGTAAGAGATGT GGCATTCTTTCAGGAGCTGT 50.0 50.0 58.0 58.2 2237 bp 0.5 58.6
ndhD_p2 TGCGTCGGTTGTAAGAGATG GGCATTCTTTCAGGAGCTGT 50.0 50.0 58.0 58.2 2238 bp 0.5 58.6
ndhD_p3 GCGTCGGTTGTAAGAGATGT TCAGGAGCTGTATGGGCTAA 50.0 50.0 58.0 57.8 2228 bp 0.5 58.5
ndhD_p4 GCGTCGGTTGTAAGAGATGT TTCAGGAGCTGTATGGGCTA 50.0 50.0 58.0 57.8 2229 bp 0.5 58.5
ndhD_p5 TGCGTCGGTTGTAAGAGATG TCAGGAGCTGTATGGGCTAA 50.0 50.0 58.0 57.8 2229 bp 0.5 58.5