Hotspot

accD

LSC · 56049-57752 · 1704 bp

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Pi 0.0944
MarkerSeek score 81.90
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1704
variable_sites
152
pi
0.0944
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 CCGCCAGTAATAATCCCAGG GGAGGTACAAGCAAAGACCA 55.0 50.0 57.8 57.4 2341 bp 0.5 54.0
accD_p2 ATGCCGCCAGTAATAATCCC GGAGGTACAAGCAAAGACCA 50.0 50.0 57.8 57.4 2344 bp 0.5 53.9
accD_p3 GGGATGCCGCCAGTAATAAT GGAGGTACAAGCAAAGACCA 50.0 50.0 57.8 57.4 2347 bp 0.5 53.9
accD_p4 AATCTTAGCCGTTGCATCGA GGAGGTACAAGCAAAGACCA 45.0 50.0 57.7 57.4 2817 bp 0.5 53.0
accD_p5 CCGCCAGTAATAATCCCAGG CCTTTGAACTTCCCGAAAGC 55.0 50.0 57.8 57.3 1957 bp 0.5 52.9