Hotspot

rps15-trnN-GUU

IRa · 130154-136473 · 6320 bp

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Pi 0.0366
MarkerSeek score 76.40
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
IRa
length_bp
6320
variable_sites
224
pi
0.0366
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 ACGAACATCATGAGCTGTCC CCCGGAACTTCTTCGTAGTG 50.0 55.0 58.0 58.0 587-702 bp 1.0 84.5
rps15-trnN-GUU_p2 GGAAGAGATAAGAGGGCCCT CCCGGAACTTCTTCGTAGTG 55.0 55.0 57.9 58.0 484-599 bp 1.0 84.2
rps15-trnN-GUU_p3 ACGAACATCATGAGCTGTCC CGTAGTGTCTCCGTTCCATG 50.0 55.0 58.0 58.1 574-689 bp 1.0 84.0
rps15-trnN-GUU_p4 CGTGTTGTTGCATCGAAACA CCCGGAACTTCTTCGTAGTG 45.0 55.0 57.9 58.0 861-976 bp 1.0 83.9
rps15-trnN-GUU_p5 ACGAACATCATGAGCTGTCC CAATTCCCGTTCTCAACCCA 50.0 50.0 58.0 58.1 641-756 bp 1.0 83.6