Hotspot

trnL-UAG-ndhF

SSC · 125007-126600 · 1594 bp

Back to Parrya

Pi 0.438
MarkerSeek score 77.10
Primer available yes
Label trnL-UAG-ndhF

Feature Metadata

label_name
trnL-UAG-ndhF
feature_type
igs
region
SSC
length_bp
1594
variable_sites
508
pi
0.438
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.73
markerseek_score
77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ndhF_p1 TAACCACTGGGACTGGAGAG AACGGGTCGTGTAAACCAAA 55.0 45.0 58.1 58.0 2715 bp 0.5 59.2
trnL-UAG-ndhF_p2 TAACCACTGGGACTGGAGAG ACGGGTCGTGTAAACCAAAA 55.0 45.0 58.1 58.0 2714 bp 0.5 59.2
trnL-UAG-ndhF_p3 TAACCACTGGGACTGGAGAG CCACATACGTCGGAGGTTTT 55.0 50.0 58.1 57.9 2325 bp 0.5 58.8
trnL-UAG-ndhF_p4 TCCTAAGAGCAGCGTGTCTA AACGGGTCGTGTAAACCAAA 50.0 45.0 57.9 58.0 2149 bp 0.5 58.7
trnL-UAG-ndhF_p5 TCCTAAGAGCAGCGTGTCTA ACGGGTCGTGTAAACCAAAA 50.0 45.0 57.9 58.0 2148 bp 0.5 58.7