Hotspot

ndhD-ccsA

SSC · 123615-123837 · 223 bp

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Pi 0.41
MarkerSeek score 77.30
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
223
variable_sites
86
pi
0.41
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 TTATCGATGGCTTCCCTTGC CTCTCCAGTCCCAGTGGTTA 50.0 55.0 58.0 58.1 1170 bp 0.5 59.2
ndhD-ccsA_p2 CCCTGGTTGATGGTAGTTGG CTCTCCAGTCCCAGTGGTTA 55.0 55.0 57.9 58.1 1479 bp 0.5 58.5
ndhD-ccsA_p3 TTATCGATGGCTTCCCTTGC GGCATTCTTTCAGGAGCTGT 50.0 50.0 58.0 58.2 841 bp 0.5 58.4
ndhD-ccsA_p4 TTATCGATGGCTTCCCTTGC TCAGGAGCTGTATGGGCTAA 50.0 50.0 58.0 57.8 832 bp 0.5 58.3
ndhD-ccsA_p5 TTATCGATGGCTTCCCTTGC TTCAGGAGCTGTATGGGCTA 50.0 50.0 58.0 57.8 833 bp 0.5 58.3