Hotspot

ndhE-psaC

SSC · 121473-121713 · 241 bp

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Pi 0.498
MarkerSeek score 76.60
Primer available yes
Label ndhE-psaC

Feature Metadata

label_name
ndhE-psaC
feature_type
igs
region
SSC
length_bp
241
variable_sites
111
pi
0.498
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-psaC_p1 TCTGTGGCTCGTCAACAAAA ACATCTCTTACAACCGACGC 45.0 50.0 58.0 58.0 976 bp 0.5 59.7
ndhE-psaC_p2 TTCTGTGGCTCGTCAACAAA ACATCTCTTACAACCGACGC 45.0 50.0 58.0 58.0 977 bp 0.5 59.7
ndhE-psaC_p3 TTTCTGTGGCTCGTCAACAA ACATCTCTTACAACCGACGC 45.0 50.0 58.0 58.0 978 bp 0.5 59.7
ndhE-psaC_p4 TCTGTGGCTCGTCAACAAAA CATCTCTTACAACCGACGCA 45.0 50.0 58.0 58.0 975 bp 0.5 59.7
ndhE-psaC_p5 TTCTGTGGCTCGTCAACAAA CATCTCTTACAACCGACGCA 45.0 50.0 58.0 58.0 976 bp 0.5 59.7