Hotspot

rbcL-accD

LSC · 55098-56048 · 951 bp

Back to Parrya

Pi 0.102
MarkerSeek score 83.70
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
951
variable_sites
91
pi
0.102
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 TTGAATGCTACTGCGGGTAC GTTTCCTCCAGCTTCTCGAG 50.0 55.0 58.0 58.0 2252 bp 0.5 59.8
rbcL-accD_p2 TGCAATGCACGCTGTTATTG GTTTCCTCCAGCTTCTCGAG 45.0 55.0 58.0 58.0 2085 bp 0.5 59.8
rbcL-accD_p3 TCATGGTATGCACTTCCGTG GTTTCCTCCAGCTTCTCGAG 50.0 55.0 58.0 58.0 2052 bp 0.5 59.8
rbcL-accD_p4 AATGAGGGACGTGATCTTGC GTTTCCTCCAGCTTCTCGAG 50.0 55.0 58.0 58.0 1676 bp 0.5 59.7
rbcL-accD_p5 CTTAACAGGGGGATTCACCG GTTTCCTCCAGCTTCTCGAG 55.0 55.0 58.0 58.0 2163 bp 0.5 59.7