Hotspot

rpl16

LSC · 84720-86133 · 1414 bp

Back to Parrotia

Pi 0.00283
MarkerSeek score 60.50
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1414
variable_sites
4
pi
0.00283
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 AGACCCCTTTTCTTTGGTTCT TGAGCAATTCTATAGGGCTGA 42.9 42.9 57.0 56.2 1494-1496 bp 1.0 42.3
rpl16_p2 AGACCCCTTTTCTTTGGTTCT TGAGCAATTCTATAGGGCTGAA 42.9 40.9 57.0 57.0 1494-1496 bp 1.0 40.3
rpl16_p3 AAGACCCCTTTTCTTTGGTTCT TGAGCAATTCTATAGGGCTGA 40.9 42.9 57.7 56.2 1495-1497 bp 1.0 40.3
rpl16_p4 AGACCCCTTTTCTTTGGTTCT ATGAGCAATTCTATAGGGCTGA 42.9 40.9 57.0 56.7 1495-1497 bp 1.0 40.3
rpl16_p5 AAGACCCCTTTTCTTTGGTTCT TGAGCAATTCTATAGGGCTGAA 40.9 40.9 57.7 57.0 1495-1497 bp 1.0 40.3