Hotspot

ndhD

SSC · 120083-121603 · 1521 bp

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Pi 0.0046

MarkerSeek score 48.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 7
pi: 0.0046
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GTAATCCGGCGAGAACCATT	AGAGGACTGTGTCGGTTGTA	50.0	50.0	58.1	58.0	1940-1956 bp	1.0	80.0
ndhD_p2	GTAATCCGGCGAGAACCATT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.1	58.0	1954-1970 bp	1.0	80.0
ndhD_p3	AGACAAACAAAGTACGGCGT	AGAGGACTGTGTCGGTTGTA	45.0	50.0	58.1	58.0	1979-1995 bp	1.0	79.9
ndhD_p4	AGACAAACAAAGTACGGCGT	TCTGCTCCAAGAACAGAGGA	45.0	50.0	58.1	58.0	1993-2009 bp	1.0	79.9
ndhD_p5	AGGTGCAAATTCCGCAATTG	AGAGGACTGTGTCGGTTGTA	45.0	50.0	57.9	58.0	2124-2140 bp	1.0	79.7