Hotspot

accD

LSC · 60587-62116 · 1530 bp

Back to Parrotia

Pi 0.00261

MarkerSeek score 57.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1530
variable_sites: 4
pi: 0.00261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCATTTCTTTCAATACGCACTCG	CGGATTTTGACTTTGATTCCGA	39.1	40.9	57.8	57.3	1703 bp	1.0	40.2
accD_p2	TCATTTCTTTCAATACGCACTCG	TCGGATTTTGACTTTGATTCCG	39.1	40.9	57.8	57.3	1704 bp	1.0	40.2
accD_p3	TCCGAGTGATTGGATCTATATGC	CGGATTTTGACTTTGATTCCGA	43.5	40.9	57.7	57.3	1666 bp	1.0	40.2
accD_p4	TCCGAGTGATTGGATCTATATGC	TCGGATTTTGACTTTGATTCCG	43.5	40.9	57.7	57.3	1667 bp	1.0	40.2
accD_p5	CCGAGTGATTGGATCTATATGCT	CGGATTTTGACTTTGATTCCGA	43.5	40.9	57.7	57.3	1665 bp	1.0	40.2