Hotspot

psbT-psbN

LSC · 77868-77926 · 59 bp

Back to Parrotia

Pi 0.0169

MarkerSeek score 62.20

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 1
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	GGGTACGCCTTATATACCGC	50.0	55.0	57.6	57.7	174 bp	1.0	75.3
psbT-psbN_p2	GAGAACCGCCTAAAGTTCCA	GGGTACGCCTTATATACCGC	50.0	55.0	57.5	57.7	172 bp	1.0	74.5
psbT-psbN_p3	TCGAGAACCGCCTAAAGTTC	GATCCATTCGAGGAACACGG	50.0	55.0	57.6	58.4	126 bp	1.0	74.3
psbT-psbN_p4	GAGAACCGCCTAAAGTTCCA	GATCCATTCGAGGAACACGG	50.0	55.0	57.5	58.4	124 bp	1.0	73.5
psbT-psbN_p5	TCGAGAACCGCCTAAAGTTC	GGCAACCCTCTCAACAACTA	50.0	50.0	57.6	57.4	149 bp	1.0	73.2