Hotspot

ycf4-cemA

LSC · 63882-64791 · 910 bp

Back to Parrotia

Pi 0.00448

MarkerSeek score 63.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 4
pi: 0.00448
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.1	58.1	1012-1029 bp	1.0	80.3
ycf4-cemA_p2	AGTTGGCCTATTTCTTGCGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	1014-1031 bp	1.0	79.4
ycf4-cemA_p3	GTTGGCCTATTTCTTGCGTG	GAGATCCACCAGGGCAAAAA	50.0	50.0	57.7	58.1	1013-1030 bp	1.0	78.9
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	GAGAGAGAGATCCACCAGGG	45.0	60.0	58.1	58.3	1018-1035 bp	1.0	78.5
ycf4-cemA_p5	AGTTGGCCTATTTCTTGCGT	GAGAGAGAGATCCACCAGGG	45.0	60.0	57.8	58.3	1020-1037 bp	1.0	77.7