Hotspot

accD-psaI

LSC · 62117-62803 · 687 bp

Back to Parrotia

Pi 0.00582

MarkerSeek score 55.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 4
pi: 0.00582
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTTCACGCTTTCTTTCCTT	GGCACCAAAACAGAGGGTAA	45.0	50.0	56.9	58.0	765-771 bp	1.0	71.8
accD-psaI_p2	GCTTCACGCTTTCTTTCCTT	AAAGAAGCCATTGCCATTGC	45.0	45.0	56.9	57.9	807-813 bp	1.0	71.0
accD-psaI_p3	GCTTCACGCTTTCTTTCCTT	CCATTGCCGGAACTACTAGG	45.0	55.0	56.9	57.8	794-800 bp	1.0	69.9
accD-psaI_p4	GCTTCACGCTTTCTTTCCTTT	GGCACCAAAACAGAGGGTAA	42.9	50.0	57.6	58.0	765-771 bp	1.0	69.5
accD-psaI_p5	GCTTCACGCTTTCTTTCCTT	CCGGAACTACTAGGCCTACT	45.0	55.0	56.9	57.6	788-794 bp	1.0	69.0