Hotspot

atpB-rbcL

LSC · 57800-58540 · 741 bp

Back to Parrotia

Pi 0.00675

MarkerSeek score 62.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 5
pi: 0.00675
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	887 bp	1.0	71.9
atpB-rbcL_p2	GGGGGAAAAGCTACATCCAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.9	58.0	900 bp	1.0	71.8
atpB-rbcL_p3	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	886 bp	1.0	71.7
atpB-rbcL_p4	GGGGGAAAAGCTACATCCAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.9	58.1	899 bp	1.0	71.6
atpB-rbcL_p5	TCCAGTACCGGACCAATGAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	885 bp	1.0	71.3