Hotspot

trnT-GGU-psbD

LSC · 34437-35800 · 1364 bp

Back to Parrotia

Pi 0.00367

MarkerSeek score 53.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1364
variable_sites: 5
pi: 0.00367
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1528-1530 bp	1.0	77.6
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	CAAAAACGAAACGGTCCCTC	50.0	50.0	57.9	57.6	1527-1529 bp	1.0	76.4
trnT-GGU-psbD_p3	AGGAATTGAATCAAACGCGC	ACAAAAACGAAACGGTCCCT	45.0	45.0	57.7	58.2	1546-1548 bp	1.0	76.3
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1491-1493 bp	1.0	76.2
trnT-GGU-psbD_p5	CAAACGCGCCCTTTTAACTC	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1535-1537 bp	1.0	75.9