Hotspot

rpl32-trnL-UAG

SSC · 110675-111332 · 658 bp

Back to Paronychia

Pi 0.116

MarkerSeek score 86.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 658
variable_sites: 73
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACCGGGAATTCAAAAAGTTTCT	CTCGCACTGCTTCCTAAGAG	36.4	55.0	56.7	58.1	754-765 bp	1.0	63.5
rpl32-trnL-UAG_p2	ACCGGGAATTCAAAAAGTTTCT	CTCTCGCACTGCTTCCTAAG	36.4	55.0	56.7	58.1	756-767 bp	1.0	63.5
rpl32-trnL-UAG_p3	ACCGGGAATTCAAAAAGTTTCT	TCCTAAGAGCAGCGTGTCTA	36.4	50.0	56.7	57.9	743-754 bp	1.0	63.3
rpl32-trnL-UAG_p4	ACCGGGAATTCAAAAAGTTTCT	AGCAGCGTGTCTACCAATTT	36.4	45.0	56.7	57.8	736-747 bp	1.0	62.9
rpl32-trnL-UAG_p5	ACCGGGAATTCAAAAAGTTTCT	CTAAGAGCAGCGTGTCTACC	36.4	55.0	56.7	57.8	741-752 bp	1.0	62.7