Hotspot

ndhF-rpl32

SSC · 109888-110500 · 613 bp

Back to Paronychia

Pi 0.0881

MarkerSeek score 82.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 613
variable_sites: 49
pi: 0.0881
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAAGGGT	TTTGGAAATAGAAGTACGTTTTT	50.0	26.1	57.1	52.9	694-701 bp	1.0	49.6
ndhF-rpl32_p2	CTGGAAGTGGAATGAAGGGT	TTTTGGAAATAGAAGTACGTTTT	50.0	26.1	57.1	52.9	695-702 bp	1.0	49.6
ndhF-rpl32_p3	CTGGAAGTGGAATGAAGGGT	TTTTTGGAAATAGAAGTACGTTT	50.0	26.1	57.1	52.9	696-703 bp	1.0	49.6
ndhF-rpl32_p4	CTGGAAGTGGAATGAAGGGT	TTTTGGAAATAGAAGTACGTTTTT	50.0	25.0	57.1	53.7	695-702 bp	1.0	49.6
ndhF-rpl32_p5	CTGGAAGTGGAATGAAGGGT	TTTTTGGAAATAGAAGTACGTTTT	50.0	25.0	57.1	53.7	696-703 bp	1.0	49.6