Hotspot

psbE-petL

LSC · 62364-63490 · 1127 bp

Back to Paronychia

Pi 0.0576

MarkerSeek score 80.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1127
variable_sites: 62
pi: 0.0576
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	ACCGAAATAACTAGTTAGAGTAAGCA	50.0	34.6	57.8	57.9	1147-1181 bp	1.0	47.7
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	ACCGAAATAACTAGTTAGAGTAAGCA	55.0	34.6	58.2	57.9	1142-1176 bp	1.0	47.7
psbE-petL_p3	CAACCCGCAATGAATAGGGA	ACCGAAATAACTAGTTAGAGTAAGCA	50.0	34.6	57.9	57.9	1224-1258 bp	1.0	47.2
psbE-petL_p4	ACGAATAACCAACCCGCAAT	ACCGAAATAACTAGTTAGAGTAAGCA	45.0	34.6	58.2	57.9	1233-1267 bp	1.0	47.2
psbE-petL_p5	CGAATAACCAACCCGCAATG	ACCGAAATAACTAGTTAGAGTAAGCA	50.0	34.6	57.8	57.9	1232-1266 bp	1.0	47.2