Hotspot

psaA-pafI

LSC · 39929-40705 · 777 bp

Back to Paronychia

Pi 0.0728

MarkerSeek score 79.70

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 777
variable_sites: 52
pi: 0.0728
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	CAAGTAGTAGTTTCGGGGCC	ATCAAGCCGCTGAGTATTGG	55.0	50.0	58.0	58.0	1009-1026 bp	1.0	87.9
psaA-pafI_p2	CCAAGTAGTAGTTTCGGGGC	ATCAAGCCGCTGAGTATTGG	55.0	50.0	58.0	58.0	1010-1027 bp	1.0	87.9
psaA-pafI_p3	GGCACTAGGTCCAATGTGAG	ATCAAGCCGCTGAGTATTGG	55.0	50.0	58.0	58.0	1211-1228 bp	1.0	87.4
psaA-pafI_p4	CAAGTAGTAGTTTCGGGGCC	GAACAGGCCATTAGACAGGG	55.0	55.0	58.0	58.0	1058 bp	0.5	59.5
psaA-pafI_p5	CCAAGTAGTAGTTTCGGGGC	GAACAGGCCATTAGACAGGG	55.0	55.0	58.0	58.0	1059 bp	0.5	59.5