Hotspot

trnT-GGU-psbD

LSC · 30048-30981 · 934 bp

Back to Paronychia

Pi 0.0696

MarkerSeek score 79.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 934
variable_sites: 61
pi: 0.0696
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	AAATCCCCTTGTGCTTCAGG	55.0	50.0	58.1	58.1	1297-1483 bp	1.0	85.9
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	AAATCCCCTTGTGCTTCAGG	50.0	50.0	58.1	58.1	1300-1486 bp	1.0	85.7
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CGCAATTGAACAGATCGAGC	55.0	50.0	58.1	57.9	1411-1597 bp	1.0	85.4
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CGCAATTGAACAGATCGAGC	50.0	50.0	58.1	57.9	1414-1600 bp	1.0	85.2
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	AGCGGCTACACCAAAACTAG	55.0	50.0	58.1	57.9	1526-1712 bp	1.0	84.9