Hotspot

trnE-UUC-trnT-GGU

LSC · 29536-29975 · 440 bp

Back to Paronychia

Pi 0.0837

MarkerSeek score 79.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 440
variable_sites: 34
pi: 0.0837
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	522-550 bp	1.0	86.8
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	501-529 bp	1.0	86.5
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.7	58.1	498-526 bp	1.0	86.4
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.7	58.2	519-547 bp	1.0	85.6
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	TTGAACCGATGACTTACGCC	50.0	50.0	57.7	58.3	524-552 bp	1.0	84.5