Hotspot

trnD-GUC-trnY-GUA

LSC · 29033-29314 · 282 bp

Back to Paronychia

Pi 0.0849
MarkerSeek score 80.00
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
282
variable_sites
23
pi
0.0849
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GCGGTGCTCTAACCAATTGA TGGCAATATGTCTACGCTGG 50.0 50.0 58.6 57.8 353-403 bp 1.0 80.9
trnD-GUC-trnY-GUA_p2 GCGGTGCTCTAACCAATTGA GGCAATATGTCTACGCTGGT 50.0 50.0 58.6 57.8 352-402 bp 1.0 80.9
trnD-GUC-trnY-GUA_p3 GCGGTGCTCTAACCAATTGA GGGGACGGACTGTAAATTCG 50.0 55.0 58.6 58.4 374-424 bp 1.0 79.6
trnD-GUC-trnY-GUA_p4 GCGGTGCTCTAACCAATTGA GGGACGGACTGTAAATTCGT 50.0 50.0 58.6 57.6 373-423 bp 1.0 79.4
trnD-GUC-trnY-GUA_p5 GCGGTGCTCTAACCAATTGA GATTCTTCCTGGGTCGATGC 50.0 55.0 58.6 58.4 407-457 bp 1.0 78.6